Lymphocytes reside in organs which provide both chemical and mechanical signals. We model the mechanically restricting environment using silicon polydimethylsiloxane (PDMS) devices that confine cells to a designed height. We study how lymphocytes respond to confinement and search for the molecular mechanosensors. 

The cytoskeleton controls cell shape and mechanics. Using the recently introduced expansion and lattice light-sheet microscopy, we study the dynamic interplay between cytoskeletal organization and cell behavior.